Buffer Cleaner
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Test for Iron in water is based on colour indicator.
Procedure:
1st step : 20, 18,16,14,12,10 and 8 drops of distilled water was taken in 7 test tubes with a calibrated pipette.
2nd step : Adding 0,2,4,6,8,10 and 12 drops of Mohr’s salt solution to each of the above tubes so that the volume in each is equal to 20 mL.
3rd step : 20 drops of sample water was taken into a different tube with the same pipette.
4th step : Adding 1 drops of Hydroxylamine Hydrochloride solution into each tube.
5th step Add 3 drops of 1,10 phenanthroline solution to each tube, stir the solutions and wait for 5 minutes for the colour to develop ( reddish pink colour).
3rd step : 20 drops of sample water was taken into a different tube with the same pipette.
4th step : Adding 1 drops of Hydroxylamine Hydrochloride solution into each tube.
5th step Add 3 drops of 1,10 phenanthroline solution to each tube, stir the solutions and wait for 5 minutes for the colour to develop ( reddish pink colour).
.Compare the colour of sample water with the colour of the other tubes.
.Deduce the amount of iron from the colour chart of iron.
Result: The amount of iron in the sample is ________mg/L.
Standard chart for Iron
Test tube number |
No. of drops of Mohr’s salt solution |
No. of drops of distilled water |
Hydroxylami ne Hydrochlorid e solution (in drops) |
1,10 phenathrol ine solution (in drops) |
Amount of Iron (in ppm) |
1 |
0 |
20 |
1 |
3 |
0.0 |
2 |
2 |
18 |
1 |
3 |
0.09 |
3 |
4 |
16 |
1 |
3 |
0.18 |
4 |
6 |
14 |
1 |
3 |
0.27 |
5 |
8 |
12 |
1 |
3 |
0.36 |
7 |
10 |
10 |
1 |
3 |
0.45 |
7 |
12 |
8 |
1 |
3 |
0.54 |
Test for Iron in water:
Reagents: Standard Mohr’s salt solution 1,10 phenanthroline solution Hydroxylamine hydrochloride solution