Stains Indicator Reagents-69

Product Short Description: METHYL ORANGE (ACS GRADE)

Product Technical Specification:

C.A.S. No.	547-58-0
CI NO.	13025
M.F.	C14H14N3SO3Na
M.W.	327.34gm/mole

Specifications:

Dye Content (by Spectrophotometry)	>95%
Solubility 0.1 % (Dist. water)	Clear solution
Transition Range	pH 3.1-4.4
	Pink Orange-Yellow
Absorption maximum (pH 3.1) ?1max	501-504nm
Absorption maximum (pH 4.4) ?2max	467-471nm
Absorptivity (A1%/1cm at pH 3.1 at ?1max)	1050-1150
Absorptivity (A1%/1cm at pH 4.4 at ?2max)	750-850
Loss On Drying (110°C)	<3%

Remarks:

Colour change

The color change of a pH indicator is caused by the dissociation of the H+ ion from the indicator itself. Recall that pH indicators are not only natural dyes but also weak acids. The dissociation of the weak acid indicator causes the solution to change color.

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METHYL RED ACS GRADE

Product Short Description: METHYL RED (ACS GRADE)

Product Technical Specification:

C.A.S. No.	493-52-7
CI NO.	13020
M.F.	C15H15N3O2
M.W.	269.31gm/mole

Specifications:

Dye Content (by Spectrophotometry)	>95%
Transition Range	pH 4.2-6.2
	Pink-Yellow
Solubility 0.1% (95% EtOH)	Clear solution
Absorption maximum (pH 4.2) ?1max	523-526nm
Absorption maximum (pH 6.2) ?2max	430-434nm
Absorptivity (A1%/1cm at pH 4.2 at ?1max)	1300-1400
Absorptivity (A1%/1cm at pH 6.2 at ?2max)	700-800
Loss On Drying (110°C)	<3%

Remarks:

Physical State	Solid
Appearance	dark red to purple
Odor	none reported
pH	Not available
Vapor Pressure	Negliglble
Vapor Density	Not available
Evaporation Rate	Negligible
Viscosity	Not available
Boiling Point	Not available
Freezing/Melting Point	179-182 deg C
Decomposition Temperature	Not available
Solubility	Insoluble in water
Specific Gravity/Density	Not available
Molecular Formula	C15H15N3O2
Molecular Weight	269.30

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METHYL RED HYDROCHLORIDE

Product Short Description: METHYL RED HYDROCHLORIDE

Product Technical Specification:

C.A.S. No.	63451-28-5
CI NO.	13020
M.F.	C15H16N3O2Cl
M.W.	305.77gm/mole

Specifications:

Dye Content (by Spectrophotometry)	>90%
Solubility 0.1%(95% EtOH)	Clear solution
Transition Range	pH 4.2-6.2
	Pink-Yellow
Absorption maximum (EtOH) ?max	490-496nm
Absorptivity (A1%/1cm in Ethanol) at ?max	>550
Loss On Drying (110°C)	<3%

Remarks:

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GIEMSA STAIN

Product Short Description: GIEMSA STAIN APPLICATION: MICROSCOPICAL STAINING

Product Technical Specification:

C.A.S. No.

51811-82-6

Specifications:

Appearance	Greenish Powder
Solubility 0.1 % (MeOH)	Clear Blue Solution with Fluorescence
Absorption Maximum(in MeOH) ?1max	640-650 nm
Absorption Maximum(in MeOH) ?2max	520 - 525 nm
Absorptivity (A1%1cm in MeOH at ?1max on dried substance)	950-1150
Absorptivity (A1%1cm in MeOH at ?2max on dried substance)	600-750
Loss On Drying (110°C)	<10%
Suitability for microscopy	Passes test

Remarks:

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HYDROXY NAPHTHOL BLUE ACS GRADE

Product Short Description: HYDROXY NAPHTHOL BLUE (ACS GRADE)

Product Technical Specification:

C.A.S. No.	165660-27-5
M.F.	C20H12N2O11Na2S3
M.W.	598.50gm/mole

Specifications:

Solubility 0.1 % (Dist. Water)	Clear solution
Suitability for Calcium determination	Passes test
Absorption maximum (Ammonia buffer pH 12.0+2ml 0.05M E.D.T.A.) ?max	645-650nm
Absorptivity (A1% 1cm at ?max, in Ammonia buffer pH 12.0+2ml 0.05M E.D.T.A.)	>350
Loss On Drying (110°C)	<5%

Remarks:

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JENNER STAIN

Product Short Description: JENNER STAIN

Product Technical Specification:

C.A.S. No.

62851-42-7

Specifications:

Solubility 0.1 % (MeOH)	Clear solution
Absorption Maximum(in MeOH) ?1max	650-653nm
Absorption Maximum(in MeOH) ?2max	520-525nm
Absorptivity (A1%1cm in MeOH at ?1max on dried substance)	>1100
Absorptivity (A1%1cm in MeOH at ?2max on dried substance)	>600
Loss On Drying (110°C)	<10%

Remarks:

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LEISHMAN STAIN

Product Short Description: LEISHMAN STAIN

Product Technical Specification:

C.A.S. No.

12627-53-1

Specifications:

APPEARANCE	BLACKISH BLUE POWDER
Solubility 0.1 % (MeOH)	CLEAR BLUE SOLUTION WITH GREEN FLUORESCENCE
Absorption Maximum(in MeOH) ?1max	640-645nm
Absorption Maximum(in MeOH) ?2max	522-525nm
Absorptivity (A1%1cm in MeOH at ?1max on dried substance)	>900
Absorptivity (A1%1cm in MeOH at ?2max on dried substance)	>650
Loss On Drying (110°C)	<8%
Suitability for microscopy	Passes test

Remarks:

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EOSIN YELLOW

Product Short Description: EOSIN YELLOW

Product Technical Specification:

C.A.S. No.	17372-87-1
CI NO.	45380
M.F.	C20H6Br4Na2O5
M.W.	691.86gm/mole

Specifications:

Appreance	RED/ORANGE POWDER
Dye Content (Spectrophotometry)	>90%
Solubility 0.1 % (Dist. Water)	Clear solution
Absorption Maximum (Dist. water) ?max	515-518nm
Absorptivity (A1%,1cm in water at ?max)	>1260
Loss On Drying (110°C)	<8%
Suitability for microscopy	Passes test

Remarks:

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ETHYL ORANGE

Product Short Description: ETHYL ORANGE

Product Technical Specification:

C.A.S. No.	62758-12-7
M.F.	C16H18N3SO3Na
M.W.	355.39gm/mole

Specifications:

Solubility 0.1 % (Dist. Water)	Clear solution
Transition Range	pH 3.0-4.8
	Red-Orange Yellow
Absorption maximum (Dist. Water) ?max	472-477nm
Absorptivity (A1% 1cm in Dist. water, at ?max)	>850
Loss On Drying (110°C)	<5%

Remarks:

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EVAN'S BLUE

Product Short Description: EVAN'S BLUE

Product Technical Specification:

C.A.S. No.	314-13-6
CI NO.	23680
M.F.	C34H24N6Na4O14S4
M.W.	960.82gm/mole

Specifications:

Dye Content (by Spectrophotometry)	>85%
Solubility 0.1 % (Dist. Water)	Clear solution
Absorption maximum (Dist. water) ?max	605-611nm
Absorptivity (A1% 1cm in water, at ?max, on dried substance)	>800
Loss On Drying (110°C)	<10%
Suitability for microscopy	Passes test

Remarks:

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