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Physical and Chemical properties:
Synonyms: 4-(dimethylamino)benzaldehyde; 4 dimethylaminobenzenecarbonal; Erlichs Reagent
CAS No.: 100-10-7
Molecular Weight: 149.19
Chemical Formula: (CH3)2NC6H4CHO
Molecular formula C9H11NO
Appearance White crystalline powder
Purity By GC, using Capillary Column : 99.42%
Melting Point 72 -75 C
Boiling Point 176 – 177 C
Solubility : Freely Soluble in Methanol, Benzene, Ether and diluted Hydrochloric Acid
Indole TEST : ( Indole produced by microorganisms )
The ability to degrade amino acid to identify end products to differentiate bacteria.Indole can be detected to the culture growth ( Pure bacterial culture must be grown in sterile tryptophan or peptone broth for 24-48 hours before performing the test. ) by reaction with 5 drops of Kovac’s reagent to produce a red or red violet colour.
KOVAC’S Reagent - ( para-Dimethylaminobenzaldehyde in isoamyl alcohol and Concentrated hydrochloric acid )
Ehrlich Reagent - ( para-Dimethylaminobenzaldehyde in ethyl alcohol and Concentrated hydrochloric acid )
INDOLE TEST is suitable for
Indole-Positive Bacteria that test positive for cleaving indole from tryptophan include: Aeromonas hydrophilia, Aeromonas punctata, Bacillus alvei, most Citrobacter sp., Edwardsiella sp., Escherichia coli,Flavobacterium sp., Haemophilus oxytoca, Proteus sp. (not P. shigelloides, Pasteurella pneumotropica, Streptococcus faecalis and Vibrio species. Bacteria which give negative results for the indole test include: Actinobacillus spp., Aeromonas salmonicida, Alcaligenes sp., most Bacillus sp., Bordetella sp., Enterobacter sp.,Lactobacillus spp., most Haemophilus sp., most Klebsiella sp., Neisseria sp., Pasteurella haemolytica, Pasteurella ureae, Proteus mirabilis, Pseudomonas sp., Salmonella sp., Serratia sp., Yersinia sp.50, 51 Kitasato's discovery that Escherichia coli could be distinguished from Klebsiella species (Aerobacter aerogenes) by virtue of its ability to produce indole initiated the detection of indole production as an accepted analytical tool for the bacteriologist
MECHANISM :
INDOLE TEST chemical concept :::
Principally this test is based on to determine the ability of microbe to degrade the AMINO ACID TRYPTOPHAN.
Tryptophan ——(Tryptophanase)——> Indole + Pyruvic Acid + Ammonia
p-dimethylaminobenzaldehyde ( with Hcl and isoamyl alcohol ) + Indole —-------------> Quinoidal Red - or - Violet Compound
Result : Development of Cherry Red Colour at the interface of the reagent and the broth, within seconds after adding the Kovacs reagent indicate the presence of indole and the test is positive. If no colour change is observed, then the test is negative and so organisms are not capable of producing tryptophanase.
Help full link to test bacterials growth in drinking water http://www.rxmarine.com/H2S-vial-Bacteriological-Kit-manufacturer-supplier-test-kit , This test is very simple and can be tested by unskilled / skilled helper both.
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The products that we manufacture and supply are as per the following standards:
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Physical
Appearance: Dark grey/black powder |
Chemical
Iodine Value : 1000 mg / Gram |
Activated carbon manufacturer, supplier and exporter. We are manufacturing Granular Activated Carbon with various Iodine Adsorption value (mg. /gm.): 450 IV - 1200 IV
We manufacture and export Activated Carbon to our clients that are formulated using premium quality chemicals and according to the international quality standards. These are used for the reduction of the impurities related to taste, odour and colour inorganic chemicals. Our ranges of products are fully tested by quality analysts to ensure that the defect free range is supplied to our customers. We offer these at cost effective prices.
Sodium bromide is an inorganic compound with the formula NaBr. It is a widely used source of the bromide ion and has many applications.
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Sodium bromide is a strong salt solution. Protective clothing, rubber gloves and eye protection are recommended. Rubber safety boots should also be worn in work areas, since sodium bromide can damage leather. This product should be handled in areas with proper ventilation. For proper disposal guidelines for sodium bromides wastes, consult the appropriate local regulatory authorities.
