CRYSTAL VIOLET STAINING SOLUTION GRAMS
- Part number
- RXSOL-60-6604-368
- Category
- Lab Chemical
- Brand
- RXSOL
Overview
Preparation of solutions Gram Crystal Violet Solution: • Dissolve 20 g of crystal violet in 100 ml of ethanol to make a crystal violet stock solution. Similarly, dissolve 1 g of ammonium oxalate in 100 ml of water to make an oxalate stock solution.
Applications
Application: Crystal violet is also a mitotic agent and mutagen. It has been used for topical treatment in creams against fungal and bacterial infections. Its been used against some pathogenic fungi such as Candida species and some gram-positive bacteria, e.g. Staphylococcus species.
Dosage and directions
Preparation of solutions Gram Crystal Violet Solution: • Dissolve 20 g of crystal violet in 100 ml of ethanol to make a crystal violet stock solution.
Characteristics and notes
Gram positive bacteria stain violet due to the presence of a thick layer of peptidoglycan in their cell walls, which retains the crystal violet these cells are stained with.
Safety and SDS
Read the safety data sheet before handling. Wear suitable gloves and eye protection.
View safety data sheet
| Product Name |
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| Product Type | CRYSTAL VIOLET STAINING SOLUTION (GRAMS) | ||
| Revised date |
Company Details:
RX MARINE INTERNATIONAL
105, A wing , BSEL , TECH PARK.
VASHI ,NEW BOMBAY 400703 INDIA
Stock Point : Mumbai, Kandla, Chennai, Visakhapatnam, Kolkata, Fujairah, Muscat Barka
| Phone | +91 22 27815541 / 42 |
| Fax | +91 22 2781 1318 :::AOH :0091 9821214367 |
| rxsolchemicals@gmail.com |
2.1. Substances : CRYSTAL VIOLET STAINING SOLUTION (GRAMS)
Substance name Contents CAS No
Water 78 7732-18-5
4-[4,4'-bis(dimethylamino) 2 548-62-9
Ammonium Oxalate Purified 99% 0.8 6009-70-7
Ethanol 20 64-17-5
Substance.
Contains no other components or impurities which will influence the classification of the
product.
3.1. Classification of the substance or mixture
Classification according to Regulation (EC) No. 1272/2008
[CLP]Mixtures/Substances: SDS EU 2015: According to
Regulation (EU) 2015/830 (REACH Annex II)
Flammable liquids, H226
Category 3
Serious eye damage/eye irritation, H319
Category 2
Carcinogenicity, H351
Category 2
Hazardous to the aquatic environment — Chronic Hazard, H412
Category 3
Adverse physicochemical, human health and environmental effects
No additional information available
3.2. Label elements
Labelling according to Regulation (EC) No. 1272/2008 [CLP] Extra labelling to
displayExtra classification(s) to display
Symbol(s) : T : Very toxic
Highly flammable
R Phrase(s) : R10: Flammable.
R35: Cause severe burns.
S Phrase(s) : S23: Do not breath gas/fumes/vapour/spray (Appropriate wording to be
specified by the manufacturer).
S26: In the case of contact with eyes, rinse immediately with plenty of water and seek
medical advise.
S45: In case of accident or if you feel unwell, seek medical advice immediately (show the
label where possible).
Hazard pictograms :
Signal word : Warning
Hazard statements : H226 - Flammable liquid and vapour.
H319 - Causes serious eye irritation.
H351 - Suspected of causing cancer.
H412 - Harmful to aquatic life with long lasting effects.
Precautionary statements : P273 - Avoid release to the environment.
P281 - Use personal protective equipment as required.
P305+P351+P338 - IF IN EYES: Rinse cautiously with water for several minutes.
Remove contact lenses, if present and easy to do. Continue rinsing.
3.3. Other hazards
No additional information available
.
4.1. Description of first aid measures
First-aid measures after inhalation : Remove person to fresh air and keep comfortable for
breathing. Immediately call a POISON CENTER/doctor.
First-aid measures after skin contact : Take off immediately all contaminated clothing.
Wash with plenty of water/…. Immediately call a POISON CENTER/doctor.
First-aid measures after eye contact : Rinse cautiously with water for several minutes.
Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a
POISON CENTER/doctor.
First-aid measures after ingestion : Rinse mouth. Do NOT induce vomiting. Immediately
call a POISON CENTER/doctor.
4.2. Most important symptoms and effects, both acute and delayed
Symptoms/effects after eye contact : Causes serious eye irritation
. Chronic symptoms : May cause cancer.
4.3. Indication of any immediate medical attention and special treatment needed
Treat symptomatically.
5.1. Extinguishing media
Suitable extinguishing media : Carbon dioxide. Dry powder. Foam. Water spray.
Unsuitable extinguishing media : Do not use a heavy water stream.
5.2. Special hazards arising from the substance or mixture
Fire hazard : Flammable liquid and vapour.
Explosion hazard : Heating may cause a fire or explosion.
5.3. Advice for firefighters
Protection during firefighting : Do not enter fire area without proper protective
equipment, including respiratory protection.
6.1. Personal precautions, protective equipment and emergency procedures
6.1.1. For non-emergency personnel
Emergency procedures : Evacuate unnecessary personnel.
6.1.2. For emergency responders
Protective equipment : Use personal protective equipment as required.
Emergency procedures : Ventilate area.
6.2. Environmental precautions
Harmful to aquatic life with long lasting effects.
6.3. Methods and material for containment and cleaning up
Methods for cleaning up : Collect spillage. On land, sweep or shovel into suitable
containers.
6.4. Reference to other sections
No additional information available
7.1. Precautions for safe handling
Precautions for safe handling : Do not breathe vapours. Avoid contact with skin and eyes.
Provide good ventilation in process area to prevent formation of vapour.
Hygiene measures : Do not eat, drink or smoke when using this product. Wash hands and
other exposed areas with mild soap and water before eating, drinking or smoking and
when leaving work.
7.2. Conditions for safe storage, including any incompatibilities
Storage conditions : Store in a well-ventilated place. Keep container tightly closed.
7.3. Specific end use(s)
No additional information available
8.1. Control parameters
No additional information available
8.2. Exposure controls
Hand protection : Protective gloves
Eye protection : Chemical goggles or face shield
Skin and body protection : Wear suitable protective clothing
Respiratory protection : [In case of inadequate ventilation] wear respiratory protection.
9.1. Information on basic physical and chemical properties
Odour threshold : No data available
pH : 4.6
Relative evaporation rate (butylacetate=1) : No data available
Melting point : No data available
Freezing point : No data available
Boiling point : No data available
Flash point : 37.8 °C
Auto-ignition temperature : No data available
Decomposition temperature : No data available
Flammability (solid, gas) : Flammable
Vapour pressure : No data available
Relative vapour density at 20 °C : No data available
Relative density : No data available
Solubility : No data available
Log Pow : No data available
Viscosity, kinematic : No data available
Viscosity, dynamic : No data available
Explosive properties : No data available
Oxidising properties : No data available
Explosive limits : No data available
9.2. Other information
No additional information available
10.1. Reactivity Flammable liquid and vapour. 10.2. Chemical stability Stable under normal conditions. 10.3. Possibility of hazardous reactions No additional information available 10.4. Conditions to avoid Open flame. Heat. Sparks. Overheating. 10.5. Incompatible materials No additional information available 10.6. Hazardous decomposition products Thermal decomposition generates : Corrosive vapours.
11.1. Information on toxicological effects
Acute toxicity • Inhalation : Based on available data, the classification criteria are not met.
• Dermal : Based on available data, the classification criteria are not met.
• Ingestion : Based on available data, the classification criteria are not met.
Corrosion : Based on available data, the classification criteria are not met.
Irritation : Based on available data, the classification criteria are not met.
Sensitization : Based on available data, the classification criteria are not met.
Mutagenicity : Based on available data, the classification criteria are not met.
Carcinogenicity : Based on available data, the classification criteria are not met.
Toxic for reproduction : Based on available data, the classification criteria are not met.
STOT-single exposure : Based on available data, the classification criteria are not met.
STOT-repeated exposure : Based on available data, the classification criteria are not met.
Aspiration hazard : Based on available data, the classification criteria are not met.
12.1. Toxicity
Toxicity information : Not established.
12.2. Persistence - degradability
Persistence - degradability : Biodegradable.
12.3. Bioaccumulative potential
Bioaccumulative potential : Not established.
12.4. Mobility in soil
Mobility in soil : Not established.
12.5. Results of PBT and vPvB assessment
Results of PBT and vPvB assessment : The substance does not fulfil the criteria
to be identified as PBT substance or vPvB substance
according to Annex XIII of Regulation REACH.
12.6. Other adverse effects
Environmental precautions : Avoid release to the environment.
13.1. Waste treatment methods
General : Avoid release to the environment. Dispose in a safe manner
in accordance with local/national regulations.
14.1. Land transport (ADR-RID)
General information : Not regulated.
14.2. Sea transport (IMDG) [English only]
General information : Not regulated.
14.3. Air transport (ICAO-IATA) [English only]
General information : Not regulated.
15.1. Safety, health and environmental regulations/legislation specific for the substance or mixture
Safety, health and environmental : Ensure all national/local regulations are observed.
regulations/legislation specific for the substance or mixture
REACH Restrictions - Annex XVII : The components of this product are not subject to restrictions.
REACH Authorisation - Annex XIV : The components of this product are not subject to authorization.
15.2. Chemical Safety Assessment
Chemical Safety Assessment : It has not been carried out.
Abbreviations and acronyms : PBT: persistent, bioaccumulative and toxic.
vPvB: very persistent and very bioaccumulative
Sources of key data used : REGULATION (EC) No 1272/2008 OF THE EUROPEAN
PARLIAMENT AND OF THE COUNCIL of 16 December 2008 on classification,
labelling and packaging of substances and mixtures, amending and repealing Directives
67/548/EEC and 1999/45/EC, and amending Regulation (EC) No 1907/2006
Further information : None.
In accordance with REACH Regulation (CE) Nº 1907/2006 and with CLP Regulation
(CE) Nº 1272/2008
DISCLAIMER OF LIABILITY :The information in this SDS was obtained from sources which we believe are reliable. However, the information is provided without any warranty, express or implied, regarding its correctness. The conditions or methods of handling, storage, use or disposal of the product are beyond our control and may be beyond our knowledge. For this and other reasons, we do not assume responsibility and expressly disclaim liability for loss, damage or expense arising out of or in any way connected with the handling, storage, use or disposal of the product. This MSDS was prepared and is to be used only for this product. If the product is used as a component in another product, this MSDS information may not be applicable.
Availability
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