RXSOL CHEMO PHARMA International Navi Mumbai, India

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Stains Indicator Reagents

BIEBRICH SCARLET

Biebrich scarlet: an acid azo dye that is used as a biological stain for cytoplasm. Fluorescent labeling of white cells in tissue samples
Part number
RXSOL-69-4196-990
Brand
RXSOL
BIEBRICH SCARLET

Overview

BIEBRICH SCARLET excellent quality manufacturer, exporter and supplier. BIEBRICH SCARLET is Azo organosulfur dye which is developed by German chemist Rudolf Nietzki.

Technical specifications

C.A.S. No. 4196-99-0
CI NO. 26905
M.F. C22H14N4Na2O7S2
M.W. 556.49gm/mole

Specifications:

Dye Content (by Spectrophotometry) >70%
Solubility 0.1% (Dist. Water) Clear solution
Absorption Maximum (Dist. Water) ?max 506-510nm
Absorptivity (A1%/1cm,in water at ?max on dried substance) 530-740
Loss On Drying (110°C) <15%
Suitability for microscopy Passes test

Applications

APPLICATION: FOR MICROSCOPICAL STAINING 
Biebrich scarlet: an acid azo dye that is used as a biological stain for cytoplasm.
Fluorescent labeling of white cells in tissue samples
The stain, Biebrich scarlet, is photo fixing and is especially useful for identifying eosinophils by binding to their cytoplasmic granules in tissue samples from 6 to 60 microns in thickness. It also stains granules of macrophage and granular lymphocytes.

Dosage and directions

BIEBRICH SCARLET is Soluble in water, ethanol

Characteristics and notes

Appearance : Red to brown powder
Solubility : 20 ppm in methanol yields clear solution
Absorption maxima : 500 - 510 nm (20 ppm in methanol)
Loss on drying (at 110°C, 1hr) : <= 15.0%
Appearance : Red to brown powder
Solubility : 20 ppm in methanol yields clear solution
Absorption maxima : 500 - 510 nm (20 ppm in methanol)
Loss on drying (at 110°C, 1hr) : <= 15.0%
Synonym  : Ponceau BS; Acid Red 66 

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