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Lab Chemical

DIAZO REAGENT B For bilirubin determination

Part number
RXSOL-60-6604-426
Category
Lab Chemical
Brand
RXSOL
RXSOL

Overview

Diazo reagent composed of diazo A and diazo B, as above described, combines with bilirubin to give a reddish purple compound known as azobilin. The intensity of this color can be measured in a colorimeter for a determination of the bilirubin present in the serum.

Applications

Diazo compounds are used as precursors to carbenes, which are generated by thermolysis or photolysis, for example in the Wolff rearrangement. As such they are used in cyclopropanation for example in the reaction of ethyl diazoacetate with styrene.

Characteristics and notes

The most widely used method for bilirubin concentration determination is the diazo method, which measures the color of azobilirubin. The vanadate oxidase method is based on oxidation of bilirubin to biliverdin by vanadate.

Safety and SDS

Read the safety data sheet before handling. Wear suitable gloves and eye protection.

View safety data sheet
Product Name
RXSOL-60-6604-426
Product Type DIAZO REAGENT B 
Revised date  

Company Details:

RX MARINE INTERNATIONAL
105, A wing , BSEL , TECH PARK.
VASHI ,NEW BOMBAY 400703 INDIA

Stock Point : Mumbai, Kandla, Chennai, Visakhapatnam, Kolkata, Fujairah, Muscat Barka

Phone +91 22 27815541 / 42
Fax +91 22 2781 1318 :::AOH :0091 9821214367
Email rxsolchemicals@gmail.com
Substance name:  DIAZO REAGENT B
Substance name             Contents              CAS No     
Water                                 99%                   7732-18-5
Sodium nitrite                  0.01 - 1                7632-00-0 
Substance. Contains no other components or impurities which will influence the classification of the product.  
3.1. Classification of the substance or mixture
Classification according to Regulation (EC) No. 1272/2008 [CLP]
Mixtures/Substances: SDS EU 2015: According to Regulation (EU) 2015/830 (REACH Annex II)
Not classified
Adverse physicochemical, human health and environmental effects
No additional information available
3.2. Label elements
Labelling according to Regulation (EC) No. 1272/2008 [CLP]
Extra labelling to displayExtra classification(s) to display
Symbol(s) : Xi : Irritation.
Xn: Harmful     
R Phrase(s) : R20/22:  Harmful by inhalation and if swallowed.
R37/38: Irritating to respiratory system and skin.
S Phrase(s) : S24/25 : Avoid contact with skin and eyes.
No labelling applicable
3.3. Other hazards
No additional information available 
4.1. Description of first aid measures
First-aid measures after inhalation : Assure fresh air breathing.
Allow the victim to rest. First-aid measures after skin contact : Wash with plenty of water/….
First-aid measures after eye contact : Rinse immediately with plenty of water.
First-aid measures after ingestion : Rinse mouth.
4.2. Most important symptoms and effects, both acute and delayed
No additional information available
4.3. Indication of any immediate medical attention and special treatment needed
Treat symptomatically. 
5.1. Extinguishing media
Suitable extinguishing media : Carbon dioxide. Dry powder. Foam. Water spray.
Unsuitable extinguishing media : Do not use a heavy water stream.
5.2. Special hazards arising from the substance or mixture
No additional information available
5.3. Advice for firefighters
Protection during firefighting : Do not enter fire area without proper
protective equipment, including respiratory protection. 
6.1. Personal precautions, protective equipment and emergency procedures
6.1.1. For non-emergency personnel
Emergency procedures : Evacuate unnecessary personnel.
6.1.2. For emergency responders
Protective equipment : Use personal protective equipment as required.
6.2. Environmental precautions
Avoid release to the environment.
6.3. Methods and material
for containment and cleaning up Methods for cleaning up : Collect spillage.
On land, sweep or shovel into suitable containers.
6.4. Reference to other sections
No additional information available 
7.1. Precautions for safe handling
Precautions for safe handling : Avoid contact with skin and eyes.
Do not breathe vapours. Hygiene measures : Wash hands and other exposed
areas with mild soap and water before eating, drinking or smoking and when leaving work.
7.2. Conditions for safe storage, including any incompatibilities
Storage conditions : Store in a well-ventilated place. Keep container tightly closed.
7.3. Specific end use(s)
No additional information available 
8.1. Control parameters
No additional information available
8.2. Exposure controls
Hand protection : Protective gloves
Eye protection : Chemical goggles or safety glasses
Skin and body protection : Wear suitable protective clothing
Respiratory protection : [In case of inadequate ventilation] wear respiratory protection. 
9.1. Information on basic physical and chemical properties
Odour threshold : No data available
pH : No data available
Relative evaporation rate (butylacetate=1) : No data available
Melting point : No data available
Freezing point : No data available
Boiling point : No data available
Flash point : No data available
Auto-ignition temperature : No data available
Decomposition temperature : No data available
Flammability (solid, gas) : No data available
Vapour pressure : No data available
Relative vapour density at 20 °C : No data available
Relative density : No data available
 Solubility : No data available
Log Pow : No data available
Viscosity, kinematic : No data available
Viscosity, dynamic : No data available
Explosive properties : No data available
Oxidising properties : No data available
Explosive limits : No data available
9.2. Other information
No additional information available 
10.1. Reactivity
No additional information available
10.2. Chemical stability
Stable under normal conditions.
10.3. Possibility of hazardous reactions
No additional information available
10.4. Conditions to avoid
Air contact. Direct sunlight. Overheating.
10.5. Incompatible materials
No additional information available
10.6. Hazardous decomposition products
No additional information available 
11.1. Information on toxicological effects
Acute toxicity : Not classified
Skin corrosion/irritation : Not classified
Serious eye damage/irritation : Not classified
Respiratory or skin sensitisation : Not classified
Germ cell mutagenicity : Not classified
Carcinogenicity : Not classified
Reproductive toxicity : Not classified
STOT-single exposure : Not classified
STOT-repeated exposure : Not classified
Aspiration hazard : Not classified 
12.1. Toxicity 
No additional information available
12.2. Persistence and degradability
No additional information available
12.3. Bioaccumulative potential
No additional information available
12.4. Mobility in soil
No additional information available
12.5. Results of PBT and vPvB assessment
No additional information available
12.6. Other adverse effects
No additional information available 
13.1. Waste treatment methods
No additional information available 
In accordance with ADR / RID / IMDG / IATA / ADN
14.1. UN number
UN-No. (ADR) : Not applicable
UN-No. (IMDG) : Not applicable
UN-No. (IATA) : Not applicable
UN-No. (ADN) : Not applicable
UN-No. (RID) : Not applicable
14.2. UN proper shipping name
Proper Shipping Name (ADR) : Not applicable
Proper Shipping Name (IMDG) : Not applicable
Proper Shipping Name (IATA) : Not applicable
Proper Shipping Name (ADN) : Not applicable
Proper Shipping Name (RID) : Not applicable
14.3. Transport hazard class(es)
ADR- Transport hazard class(es) (ADR) : Not applicable
IMDG- Transport hazard class(es) (IMDG) : Not applicable
IATA- Transport hazard class(es) (IATA) : Not applicable
ADN- Transport hazard class(es) (ADN) : Not applicable
RID- Transport hazard class(es) (RID) : Not applicable
14.4. Packing group
Packing group (ADR) : Not applicable
Packing group (IMDG) : Not applicable 
Packing group (IATA) : Not applicable
Packing group (ADN) : Not applicable
Packing group (RID) : Not applicable
14.5. Environmental hazards
Dangerous for the environment : No
Marine pollutant : No Other information : No
supplementary information available
14.6. Special precautions for user
Overland transport- No data available
Transport by sea- No data available
Air transport- No data available
Inland waterway transport- No data available
Rail transport- No data available
14.7. Transport in bulk according to Annex II of MARPOL 73/78 and the IBC Code
Not applicable 
15.1. Safety, health and environmental regulations/legislation specific for the substance or mixture
15.1.1. EU-Regulations
Contains no REACH substances with Annex XVII restrictions Contains no substance on the
REACH candidate list Contains no REACH Annex XIV substances
15.1.2. National regulations
Germany
Reference to AwSV : Water hazard class (WGK) 3, severe hazard to water (Classification according to AwSV, Annex 1)
12th Ordinance Implementing the Federal
Immission Control Act - 12.BImSchV : Is not subject of the 12. BlmSchV (Hazardous Incident Ordinance)
Netherlands
SZW-lijst van kankerverwekkende stoffen : None of the components are listed
SZW-lijst van mutagene stoffen : None of the components are listed
NIET-limitatieve lijst van voor de voortplanting giftige stoffen – Borstvoeding : None of the components are listed
NIET-limitatieve lijst van voor de voortplanting giftige stoffen – Vruchtbaarheid : None of the components are listed
NIET-limitatieve lijst van voor de voortplanting giftige stoffen – Ontwikkeling : None of the components are listed
15.2. Chemical safety assessment
No additional information available 
Abbreviations and acronyms : PBT: persistent, bioaccumulative and toxic. vPvB: very persistent and very bioaccumulative Sources of key data used : REGULATION (EC) No 1272/2008 OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 16 December 2008 on classification, labelling and packaging of substances and mixtures, amending and repealing Directives 67/548/EEC and 1999/45/EC, and amending Regulation (EC) No 1907/2006 Further information : None. In accordance with REACH Regulation (CE) Nº 1907/2006 and with CLP Regulation (CE) Nº 1272/2008
DISCLAIMER OF LIABILITY The information in this SDS was obtained from sources which we believe are reliable. However, the information is provided without any warranty, express or implied, regarding its correctness. The conditions or methods of handling,storage, use or disposal of the product are beyond our control and may be beyond our knowledge. For this and other reasons, we do not assume responsibility and expressly disclaim liability for loss, damage or expense arising out of or in any way connected with the handling, storage, use or disposal of the product. This MSDS was prepared and is to be used only for this product. If the product is used as a component in another product, this MSDS information may not be applicable. 

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Availability

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