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Lab Chemical

FIELD STAIN B FOR MICROSCOPIAL STAINING

Part number
RXSOL-60-6604-598
Category
Lab Chemical
Brand
RXSOL
RXSOL

Overview

FIELD STAIN B FOR MICROSCOPIAL STAINING This SOP should be used in conjunction with the series of other SOPs Cover the slide with 1 mL of diluted Field's stain B .

Applications

Field Stains contain methylene blue and eosin. These basic and acidic dyes induce multiple colours when applied to cells. The fixative, methanol does not allow any further change in slide. The basic component of white cells (cytoplasm) is stained by
acidic dye and they are described as eosinophilic or acidophilic.Field Stains contain methylene blue and eosin. These basic and acidic dyes induce multiple colours when applied to cells. The
fixative, methanol does not allow any further change in slide. The basic component of white cells (cytoplasm) is stained by
acidic dye and they are described as eosinophilic or acidophilic.Field Stains contain methylene blue and eosin. These basic and acidic dyes induce multiple colours when applied to cells. The
fixative, methanol does not allow any further change in slide. The basic component of white cells (cytoplasm) is stained by
acidic dye and they are described as eosinophilic or acidophilic.

Dosage and directions

Dry the blood film and immerse in methanol for 2-3 minutes.Blow off with Fields Stain A (S008) for 2-3 seconds. Wash it with distilled water, and again blow with Fields Stain B (S009) for 2-3 seconds and wash with distilled water. Dry it and observe under microscope.

Characteristics and notes

The neutral component of the cells are stained by both the dyes. This staining method is used for screening thick films of malarial parasites.

Safety and SDS

Read the safety data sheet before handling. Wear suitable gloves and eye protection.

View safety data sheet
Product Name
RXSOL-60-6604-598
Product Type FIELD STAIN B FOR MICROSCOPICAL STAINING 
Revised date  

Company Details:

RX MARINE INTERNATIONAL
105, A wing , BSEL , TECH PARK.
VASHI ,NEW BOMBAY 400703 INDIA

Stock Point : Mumbai, Kandla, Chennai, Visakhapatnam, Kolkata, Fujairah, Muscat Barka

Phone +91 22 27815541 / 42
Fax +91 22 2781 1318 :::AOH :0091 9821214367
Email rxsolchemicals@gmail.com
Substance / Preparation : Preparation.
Substance nam                     Contents                          CAS No
FIELD STAIN B :             > 1 < 5 %                           -----  Water :                                                                                           
                                          > 75 < 99 %                   7732-18-5  
3.1. Classification of the substance or mixture
Classification EC 67/548 or EC 1999/45
Not classified.
Hazard Class and Category Code(s), Regulation (EC) No 1272/2008 (CLP)
Not classified.
3.2. Label elements
Labelling EC 67/548 or EC 1999/45
Not classified.
Contains : Water - FIELD STAIN B Labelling Regulation EC 1272/2008 (CLP)
Not classified.
3.3. Other hazards
Other hazards : The substance does not fulfil the criteria to be identified as
PBT substance or vPvB substance according to Annex XIII of Regulation REACH. 
4.1. Description of first aid measures
Inhalation : Assure fresh air breathing. Allow the victim to rest.
Skin contact : Remove affected clothing and wash all exposed skin
area with mild soap and water, followed by warm water rinse.
Eye contact : Rinse immediately with plenty of water. Obtain medical attention if pain,
blinking or redness persist. Ingestion : Obtain emergency medical attention. Rinse mouth. Do NOT induce vomiting.
4.2. Most important symptoms and effects, both acute and delayed
Symptoms relating to use : Not expected to present a significant hazard under
anticipated conditions of normal use.
4.3. Indication of any immediate medical attention and special treatment needed
General information : Never give anything by mouth to an unconscious person.
If you feel unwell, seek medical advice (show the label where possible). 
5.1. Extinguishing media
Suitable extinguishing media : Foam. Dry powder. Carbon dioxide. Water spray. Sand.
Unsuitable extinguishing media : Do not use a heavy water stream.
Surrounding fires : Use water spray or fog for cooling exposed containers.
5.2. Special hazards arising from the substance or mixture
Hazardous combustion products : Under fire conditions, hazardous fumes will be present.
5.3. Advice for fire-fighters
Protection against fire : Do not enter fire area without proper protective equipment,
including respiratory protection. Special procedures : Exercise caution when fighting
any chemical fire. Avoid (reject) fire-fighting water to enter environment. 
6.1. Personal precautions, protective equipment and emergency procedures
For emergency responders : Equip cleanup crew with proper protection. Ventilate area
. For non-emergency personnel : Evacuate unnecessary personnel.
6.2. Environmental precautions
Environmental precautions : Prevent entry to sewers and public waters.
Notify authorities if product enters sewers or public waters.
6.3. Methods and material for containment and cleaning up 
Clean up methods : Soak up spills with inert solids, such as clay or diatomaceous
earth as soon as possible. Collect spillage. Store away from other materials.
6.4. Reference to other sections
See section 8. Exposure controls/personal protection
7.1. Precautions for safe handling
Handling : Wash hands and other exposed areas with mild soap and water before eat,
drink or smoke and when leaving work. Technical protective measures : Provide good
ventilation in process area to prevent formation of vapour.
7.2. Conditions for safe storage, including any incompatibilities
Storage : Keep only in the original container in a cool, well ventilated place.
Keep container closed when not in use. Storage - away from : Strong bases.
Strong acids. Sources of ignition. Direct sunlight.
7.3. Specific end use(s)
Specific end use(s) : None. 
8.1. Exposure controls
Personal protection : Avoid all unnecessary exposure.
• Respiratory protection : Wear approved mask.
• Hand protection : Wear protective gloves.
• Eye protection : Chemical goggles or safety glasses.
• Others : When using, do not eat, drink or smoke.
8.2. Control parameters
Occupational Exposure Limits : No data available. 
9.1. Information on basic physical and chemical properties
Physical state at 20 °C : Liquid.
Odour threshold : No data available.
pH value : No data available.
Melting point [°C] : No data available.
Decomposition point [°C] : N/A
Critical temperature [°C] : N/A
Auto-ignition temperature [°C] : N/A
Flammability (solid, gas) : N/A
Flash point [°C] : N/A 
Boiling point [°C] : N/A
Initial boiling point [°C] : N/A
Final boiling point [°C] : N/A
Evaporation rate : N/A
Vapour pressure [20°C] : N/A
Vapour pressure mm/Hg : N/A
Vapour density : N/A
Density [g/cm3] : N/A
Relative density, gas (air=1) : N/A
Relative density, liquid (water=1) : N/A
Solubility in water [% weight] : N/A
Solubility in water : N/A
Log Pow octanol / water at 20°C : No data available.
Solubility : N/A Viscosity
at 40°C [mm2/s] : N/A
9.2. Other information
Explosive properties : N/A
Explosion limits - upper [%] : N/A
Explosion limits - lower [%] : N/A
Oxidising properties : No data available. 
10.1. Reactivity
Reactivity : Not established.
10.2. Chemical stability
Chemical stability : Stable under
recommended storage conditions.
10.3. Possibility of hazardous reactions
Hazardous reactions : Not established.
10.4. Conditions to avoid
Conditions to avoid : Direct sunlight.
Extremely high or low temperatures.
10.5. Incompatible materials
Materials to avoid : Strong acids. Strong bases.
10.6. Hazardous decomposition products
Hazardous decomposition products : Fumes.
Carbon monoxide. Carbon dioxide. 
11.1. Information on toxicological effects Toxicity
information : The product has been not fully tested.
The calculated risk has been done under the requirements of the EU regulations. 
Acute toxicity
• Inhalation : Based on available data, the classification criteria are not met.
• Dermal : Based on available data, the classification criteria are not met.
• Ingestion : Based on available data, the classification criteria are not met.
Corrosion : Based on available data, the classification criteria are not met.
Irritation : Based on available data, the classification criteria are not met.
Sensitization : Based on available data, the classification criteria are not met.
Mutagenicity : Based on available data, the classification criteria are not met.
Carcinogenicity : Based on available data, the classification criteria are not met.
Toxic for reproduction : Based on available data, the classification criteria are not met.
STOT-single exposure : Based on available data, the classification criteria are not met.
STOT-repeated exposure : Based on available data, the classification criteria are not met.
Aspiration hazard : Based on available data, the classification criteria are not met. 
12.1. Toxicity
Toxicity information : The product has been not fully tested.
The calculated risk has been done under the requirements of the EU regulations.
12.2. Persistence - degradability
Persistence - degradability : Biodegradable.
12.3. Bioaccumulative potential
Bioaccumulative potential : Not established.
12.4. Mobility in soil
Mobility in soil : Not established.
12.5. Results of PBT and vPvB assessment
Results of PBT and vPvB assessment : The substance does
not fulfil the criteria to be identified as PBT substance or vPvB
substance according to Annex XIII of Regulation REACH.
12.6. Other adverse effects
Environmental precautions : Avoid release to the environment. 
13.1. Waste treatment methods
General : Avoid release to the environment.
Dispose in a safe manner in accordance with local/national regulations. 
14.1. Land transport (ADR-RID) 
General information : Unregulated.
14.2. Sea transport (IMDG) [English only]
General information : Unregulated.
14.3. Air transport (ICAO-IATA) [English only]
General information : Unregulated. 
15.1. Safety, health and environmental regulations/legislation specific for the substance or mixture
Safety, health and environmental : Ensure all national/local regulations are observed. regulations/legislation
specific for the substance or mixture REACH Restrictions - Annex XVII : The components of this product are
not subject to restrictions. REACH Authorisation - Annex XIV : The components of this product are not subject to authorization.
15.2. Chemical Safety Assessment
Chemical Safety Assessment : It has not been carried out. 
Abbreviations and acronyms : PBT: persistent, bioaccumulative and toxic. vPvB: very persistent and very bioaccumulative
Sources of key data used : REGULATION (EC) No 1272/2008 OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL
of 16 December 2008 on classification, labelling and packaging of substances and mixtures, amending and repealing Directives
67/548/EEC and 1999/45/EC, and amending Regulation (EC) No 1907/2006 Further information : None. In accordance with REACH
Regulation (CE) Nº 1907/2006 and with CLP Regulation (CE) Nº 1272/2008 
DISCLAIMER OF LIABILITY :The information in this SDS was obtained from sources which we believe are reliable. However, the information is provided without any warranty, express or implied, regarding its correctness. The conditions or methods of handling, storage, use or disposal of the product are beyond our control and may be beyond our knowledge. For this and other reasons, we do not assume responsibility and expressly disclaim liability for loss, damage or expense arising out of or in any way connected with the handling, storage, use or disposal of the product. This MSDS was prepared and is to be used only for this product. If the product is used as a component in another product, this MSDS information may not be applicable. 

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Availability

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