JSB STAIN SOLUTION NO 1

The JSB stain — Jaswant Singh–Bhattacharji stain — is the rapid two-solution method for diagnosing malaria in thick blood films, used throughout India's public-health laboratories. Solution No. 1 is the blue component: methylene blue oxidised with potassium dichromate in dilute sulphuric acid (polychromed), which stains the parasite chromatin and nuclei; Solution No. 2 (eosin) counterstains the cytoplasm. The thick smear is dipped in Solution 1 for 30–40 seconds, rinsed in buffered water, dipped in Solution 2 for a few seconds, rinsed, then returned to Solution 1 for 30–40 seconds and rinsed again — a result in under two minutes. RXSOL supplies both solutions ready to use for hospitals, PHCs and pathology labs.