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Lab Chemical

LEISHMANS STAINING SOLUTION

Part number
RXSOL-60-6604-822
Category
Lab Chemical
Brand
RXSOL
RXSOL

Overview

LEISHMAN STAIN - Powder dye for creating dye solution for staining blood smears.

Leishman Eosin Methylene Blue dye solution: By slowly heating in water bath (40°C), dissolve 0.a

Applications

Leishman's Stain is used generally in hematology and tropical medicine to differentiate and identify leucocytes, malarial parasites and trypanosomes. Leishman stain is used in microscopy for staining blood smears. It provides excellent stain quality.

Dosage and directions

It consists of a mixture of eosin (an acidic stain), and methylene blue (a basic stain) in alcohol and is usually diluted and buffered before use. It stains the different components of blood in a range of shades between red and blue. The similar Wright's stain is favoured by American workers.

Characteristics and notes

Atlas Leishman's stain is used for staining of blood and bone marrow. It is generally used to differentiate and identify leucocytes, malaria parasites, and trypanosomas (unicellar parasitic protozoa). It is based on a mixture of methylene blue and eosin.

Safety and SDS

Read the safety data sheet before handling. Wear suitable gloves and eye protection.

View safety data sheet
Product Name
RXSOL-60-6604-822
Product Type LEISHMAN’S STAINING SOLUTION 
Revised date  

Company Details:

RX MARINE INTERNATIONAL
105, A wing , BSEL , TECH PARK.
VASHI ,NEW BOMBAY 400703 INDIA

Stock Point : Mumbai, Kandla, Chennai, Visakhapatnam, Kolkata, Fujairah, Muscat Barka

Phone +91 22 27815541 / 42
Fax +91 22 2781 1318 :::AOH :0091 9821214367
Email rxsolchemicals@gmail.com
Substance / Preparation : Preparation.
Hazardous component(s) : This product is hazardous.
Substance name                                    Contents                        CAS No
LEISHMAN`S STAIN FOR :                   > 0.1 < 1 %                    12627-53-1
MICROSCOPY
METHANOL:                                          > 99 %                            97-56-1
3.1. Classification of the substance or mixture
Classification EC 67/548 or EC 1999/45
Classification : T; R23/24/25-R39/23/24/25
Hazard Class and Category Code(s), Regulation (EC) No 1272/2008 (CLP)
Health hazards : Specific Target Organ Toxicity - Single exposure - Category 1 -
Danger (CLP : STOT SE 1) H370
Acute toxicity, dermal - Category 3 - Danger (CLP : Acute Tox. 3) H311
Acute toxicity, Inhalation - Category 3 - Danger (CLP : Acute Tox. 3) H331
Acute toxicity, Oral - Category 3 - Danger (CLP : Acute Tox. 3) H301
3.2. Label elements
Labelling EC 67/548 or EC 1999/45
Symbol(s) : T : Toxic
R Phrase(s) : R11 : Highly flammable.
R23/24/25 : Toxic by inhalation, in contact with skin and if swallowed.
R39/23/24/25 : Toxic : danger of very serious irreversible effects through inhalation,
in contact with skin and if swallowed.
S Phrase(s) : S4 : Keep away from living quarters.
S7: Keep container tightly closed.
S16 : Keep away from source of ignition-No smoking.
S24 : Avoid contact with skin.
S28 : After contact with skin, wash immediately with
S35 : This material and its container must be disposed of in a safe way.
S36/37 : Wear suitable protective clothing and gloves.
S38 : In case of insufficient ventilation, wear suitable respiratory equipment.
S45 : In case of accident or if you feel unwell, seek medical advice immediately ( show
the label when possible).
S59 : Refer to manufacturer/supplier for information on recovery/recycling.
Labelling Regulation EC 1272/2008 (CLP)
Hazard pictograms
Signal words : Danger
Hazard statements : H301 : Toxic if swallowed.
H311 : Toxic in contact with skin.
H331 : Toxic if inhaled.
H370 : Causes damage to organ.
Precautionary statements
• Prevention : P280: Wear protective gloves, protective clothing, eye protection, face
protection.
P271: Use only outdoors or in a well-ventilated area.
P260: Do not breathe dust, fume, gas, mist, vapours, spray.
P264: Wash thoroughly after handling.
P270: Do not eat, drink or smoke when using this product.
• Response : P301+P310: IF SWALLOWED : Immediately call a POISON CENTER or
doctor.
P307+P311: IF EXPOSED : Call a POISON CENTER or doctor.
P302+P352: IF ON SKIN : Wash with plenty of soap and water.
P304+P340: IF INHALED : Remove to fresh air and keep at rest in a position
comfortable for breathing.
P363: Wash contaminated clothing before reuse.
P361: Remove immediately all contaminated clothing.
• Storage : P403+P233: Store in well-ventilated place. Keep container tightly closed.
P405: Store locked up.
• Disposal considerations : P501: Dispose of this material and its container to hazardous
or special waste collection point, in accordance with local, regional, national and/or
international regulation.
P501: Dispose of this material and its container to hazardous or special waste
collection point, in accordance with local, regional, national and/or international
regulation
3.3. Other hazards
Other hazards : The substance does not fulfil the criteria to be identified as PBT
substance or vPvB substance according to Annex XIII of Regulation REACH.
4.1. Description of first aid measures
Inhalation : Remove to fresh air and keep at rest in a position comfortable for breathing.
Specific treatment (see on this label). Immediately call a POISON CENTER or
doctor.
Skin contact : Remove affected clothing and wash all exposed skin area with mild soap
and water, followed by warm water rinse. Wash with plenty of soap and water.
Immediately call a POISON CENTER or doctor. Specific treatment (see on this label).
Remove/Take off immediately all contaminated clothing. Wash contaminated
clothing before reuse.
Eye contact : Obtain medical attention if pain, blinking or redness persist. Rinse
cautiously with water for several minutes. Remove contact lenses, if present and easy to
do. Continue rinsing.
Ingestion : Immediately call a POISON CENTER or doctor. Specific treatment (see on
this label). Do NOT induce vomiting. Rinse mouth.
4.2. Most important symptoms and effects, both acute and delayed
Symptoms relating to use : Repeated exposure to this material can result in absorption
through skin causing significant health hazard. Causes damage to organ. Danger of
serious damage to health by prolonged exposure through inhalation. Swallowing a small
quantity of this material will result in serious health hazard.
4.3. Indication of any immediate medical attention and special treatment needed
General information : Never give anything by mouth to an unconscious person. If you
feel unwell, seek medical advice (show the label where possible). Call a POISON
CENTER or doctor. Specific treatment (see on this label)
5.1. Extinguishing media
Suitable extinguishing media : Foam. Dry powder. Carbon dioxide. Water spray. Sand.
Unsuitable extinguishing media : Do not use a heavy water stream.
Surrounding fires : Use water spray or fog for cooling exposed containers.
5.2. Special hazards arising from the substance or mixture
Hazardous combustion products : Under fire conditions, hazardous fumes will be
present.
5.3. Advice for fire-fighters
Protection against fire : Do not enter fire area without proper protective equipment,
including respiratory protection.
Special procedures : Exercise caution when fighting any chemical fire. Avoid (reject)
fire-fighting water to enter environment.
6.1. Personal precautions, protective equipment and emergency procedures
For emergency responders : Equip cleanup crew with proper protection.
Ventilate area.
Avoid breathing dust, fume, gas, mist, vapours, spray.
For non-emergency personnel : Evacuate unnecessary personnel.
6.2. Environmental precautions
Environmental precautions : Prevent entry to sewers and public waters. Notify
authorities if product enters sewers or public waters.
6.3. Methods and material for containment and cleaning up
Clean up methods : Soak up spills with inert solids, such as clay or diatomaceous earth
as soon as possible. Collect spillage. Store away from other materials.
6.4. Reference to other sections
See section 8. Exposure controls/personal protection
7.1. Precautions for safe handling
Handling : Use only outdoors or in a well-ventilated area. Do not breathe dust, fume,
gas, mist, vapours, spray. Wash thoroughly after handling. Do not eat, drink or smoke
when using this product.
Technical protective measures : Provide good ventilation in process area to prevent
formation of vapour.
7.2. Conditions for safe storage, including any incompatibilities
Storage : Keep only in the original container in a cool, well ventilated place. Keep
container tightly closed.
Storage - away from : Strong bases. Strong acids. Sources of ignition. Direct sunlight.
7.3. Specific end use(s)
Specific end use(s) : None.
8.1. Exposure controls
Personal protection : Avoid all unnecessary exposure.
• Respiratory protection : In case of insufficient ventilation, wear suitable respiratory
equipment. Where exposure through inhalation may occur from use, respiratory
protection equipment is recommended.
• Hand protection : Wear protective gloves.
• Eye protection : Chemical goggles or safety glasses.
• Others : When using, do not eat, drink or smoke.
8.2. Control parameters
Occupational Exposure Limits : No data available.
9.1. Information on basic physical and chemical properties
Physical state at 20 °C : Liquid.
Odour threshold : No data available.
pH value : No data available.
Melting point [°C] : -98°C
Decomposition point [°C] : N/A
Critical temperature [°C] : No data available
. Auto-ignition temperature [°C] : No data available.
Flammability (solid, gas) : Flammable.
Flash point [°C] : No data available.
Boiling point [°C] : 65 °C
Initial boiling point [°C] : No data available.
Final boiling point [°C] : No data available.
Evaporation rate : N/A
Vapour pressure [20°C] : No data available.
Vapour pressure mm/Hg : N/A
Vapour density : N/A
Density [g/cm3] : 0,8
Relative density, gas (air=1) : N/A
Relative density, liquid (water=1) : N/A
Solubility in water [% weight] : Complete.
Solubility in water : No data available.
Log Pow octanol / water at 20°C : No data available.
Solubility : N/A
Viscosity at 40°C [mm2/s] : No data available.
9.2. Other information
Explosive properties : N/A
Explosion limits - upper [%] : No data available.
Explosion limits - lower [%] : No data available.
Oxidising properties : No data available.
10.1. Reactivity
Reactivity : Not established.
10.2. Chemical stability
Chemical stability : Stable under recommended storage conditions.
10.3. Possibility of hazardous reactions
Hazardous reactions : Not established.
10.4. Conditions to avoid
Conditions to avoid : Direct sunlight. Extremely high or low temperatures.
10.5. Incompatible materials
Materials to avoid : Strong acids. Strong bases.
10.6. Hazardous decomposition products
Hazardous decomposition products : Fumes. Carbon monoxide. Carbon dioxide.
11.1. Information on toxicological effects
Toxicity information : The product has been not fully tested. The calculated risk has
been done under the requirements of the EU regulations.
Acute toxicity
• Inhalation : Toxic if inhaled.
• Dermal : Toxic in contact with skin.
• Ingestion : Toxic if swallowed.
Corrosion : Based on available data, the classification criteria are not met.
Irritation : Based on available data, the classification criteria are not met.
Sensitization : Based on available data, the classification criteria are not met.
Mutagenicity : Based on available data, the classification criteria are not met.
Carcinogenicity : Based on available data, the classification criteria are not met.
Toxic for reproduction : Based on available data, the classification criteria are not met.
STOT-single exposure : Causes damage to organ.
STOT-repeated exposure : Based on available data, the classification criteria are not
met.
Aspiration hazard : Based on available data, the classification criteria are not met.
12.1. Toxicity
Toxicity information : The product has been not fully tested. The calculated risk has
been done under the requirements of the EU regulations.
12.2. Persistence - degradability
Persistence - degradability : Biodegradable.
12.3. Bioaccumulative potential
Bioaccumulative potential : Not established.
12.4. Mobility in soil
Mobility in soil : Not established.
12.5. Results of PBT and vPvB assessment
Results of PBT and vPvB assessment : The substance does not fulfil the criteria to be
identified as PBT substance or vPvB substance according to Annex XIII of Regulation
REACH.
12.6. Other adverse effects
Environmental precautions : Avoid release to the environment.
13.1. Waste treatment methods
General : Avoid release to the environment. Dispose in a safe manner in accordance with
local/national regulations. Dispose of this material and its container to hazardous or
special waste collection point, in accordance with local, regional, national and/or
international regulation.
Special precautions : Hazardous waste due to toxicity. 
14.1. Land transport (ADR-RID)
Proper shipping name : METHANOL
UN N° : 1230
H.I. nr : 336
ADR - Class : 3
Labelling - Transport : 3 : Flammable liquid. 6.1 : Toxic substances.
ADR - Classification code : FT1
ADR - Group : II
ADR - Packing instructions : P001
ADR - Limited Quantity : 1 L
ADR - Tunnel code : D/E : Bulk or tank carriage: Passage forbidden through tunnels of
category D and E. Other carriage: Passage forbidden through tunnels of category E.
14.2. Sea transport (IMDG) [English only]
Proper shipping name : METHANOL
UN N° : 1230
IMO-IMDG - Class or division : 3 : Flammable liquid. ( 6.1 : Toxic substances. )
IMO-IMDG - Packing group : II
IMO- IMDG - Packing instructions : P001
IMO-IMDG - Limited quantities : 1 L
IMO-IMDG - Marine pollution : No
EMS-Nr : F-E S-D
14.3. Air transport (ICAO-IATA) [English only]
Proper shipping name : METHANOL
UN N° : 1230]
IATA - Class or division : 3 : Flammable liquid. ( 6.1 : Toxic substances. )
IATA - Packing group : II
IATA - Passenger and Cargo Aircraft : ALLOWED
- Passenger and Cargo - Packing : 352
instruction
- Passenger and Cargo - Maximum : 1 L
Quantity/Packing
IATA - Cargo only : ALLOWED
- Cargo only - Packing instruction : 364
- Cargo only - Maximum Quantity/ : 60 L
Packing
IATA - Limited Quantites : 1 L
ERG-Nr : 3L
15.1. Safety, health and environmental regulations/legislation specific for the
substance or mixture
Safety, health and environmental : Ensure all national/local regulations are observed.
regulations/legislation specific for the substance or mixture
REACH Restrictions - Annex XVII : The components of this product are not subject to
restrictions.
REACH Authorisation - Annex XIV : The components of this product are not subject
to authorization.
15.2. Chemical Safety Assessment
Chemical Safety Assessment : It has not been carried out.
Abbreviations and acronyms : PBT: persistent, bioaccumulative and toxic.
vPvB: very persistent and very bioaccumulative
Sources of key data used : REGULATION (EC) No 1272/2008 OF THE EUROPEAN
PARLIAMENT AND OF THE COUNCIL of 16 December 2008 on classification,
labelling and packaging of substances and mixtures, amending and repealing Directives
67/548/EEC and 1999/45/EC, and amending Regulation (EC) No 1907/2006
Further information : None.
In accordance with REACH Regulation (CE) Nº 1907/2006 and with CLP Regulation
(CE) Nº 1272/2008
DISCLAIMER OF LIABILITY :The information in this SDS was obtained from sources which we believe are reliable. However, the information is provided without any warranty, express or implied, regarding its correctness. The conditions or methods of handling, storage, use or disposal of the product are beyond our control and may be beyond our knowledge. For this and other reasons, we do not assume responsibility and expressly disclaim liability for loss, damage or expense arising out of or in any way connected with the handling, storage, use or disposal of the product. This MSDS was prepared and is to be used only for this product. If the product is used as a component in another product, this MSDS information may not be applicable.

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