Phenol Red Indicator Solution
- Part number
- RXSOL-60-6024-100
- Category
- Lab Chemical
- Brand
- RXSOL
Overview
Phenol Red Indicator Solution is used as a pH indicator. The sodium salt of phenol red is used widely in culture media to identify changes from neutral to acidic pH values.
Technical specifications
|
Physical State |
: Liquid |
|
Color |
: Red to orange |
|
Odor |
: Odorless |
|
pH |
: 7-8 |
|
Specific Gravity |
: 1.0 |
|
Solubility in water |
: Soluble |
Applications
It is typically used in cell culture media at 11 mg/L. A solution of phenol red will have a yellow color at a pH of 6.4 or below and a red color at a pH of 8.2 and above.Phenol red in tissue culture media can act as a weak estrogen, especially with human breast cancer cells. Lipophilic impurities, not the phenol red dye itself, account for the estrogenic activity. 95-99% of these impurities can be removed from the sodium salt of phenol red with a reduction in estrogen-like activity.
Dosage and directions
Preparation Instructions
This product is a sterle filtered 0.5% solution of tissue culture grade phenol red in Dulbecco,s phosphate buffered saline without magnesium and calcium.
An indicator solution can be formed by dissolving 0.1 g of phenol red in 14.20 ml of 0.02 N NaOH and diluted to 250 ml with deionized water. Phenol red can be used to measure hydrogen peroxide in cultured macrophages in multiwell plates. Assays used 2 units of peroxidase/100 ml volume with 0.5 mM phenol red, pH 7 at 37 °C. The reaction was stopped and color developed by adding 10 ml of 1 N NaOH and reading the absorption of the oxidized phenol red at 600-610 nm. Calculations were accomplished by comparison to a standard curve of hydrogen peroxide.
Characteristics and notes
Precautions and Disclaimer :
For Laboratory Use Only. Not for drug, household or other uses.
Storage/Stability
Phenol red when added as a component to tissue culture media, can be autoclaved.
Safety and SDS
Read the safety data sheet before handling. Wear suitable gloves and eye protection.
View safety data sheet
| Product Name | RXSOL-60-6024-100 |
| Product Type | Phenol Red Indicator Solution |
Company Details:
RX MARINE INTERNATIONAL
105, A wing , BSEL , TECH PARK.
VASHI ,NEW BOMBAY 400703 INDIA
| Phone | +91 22 65113333 / 5555 / 9999 |
| Fax | +91 22 2781 1318 :::AOH :0091 9821214367 |
| rxsolchemicals@gmail.com |
| CAS# | Chemical Name: | Percent |
| 7732-18-5 | Water | >99.9 |
| 34487-61-1 | Phenol red, sodium salt | 0.02-0.1 |
Emergency Overview
Appearance: Clear, red to orange liquid
Caution. May cause eye and skin irritation.
Target Organs: Eyes, skin, respiratory system
Potential Health Effects
Eye: May cause eye irritation.
Skin: May cause skin irritation.
Ingestion: May cause gastrointestinal tract irritation.
Inhalation: May cause respiratory tract irritation. Low hazard for usual industrial handling.
Chronic: No information found.
| Eyes: | Flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid. |
| Skin: | Flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Get medical aid if irritation develops. Wash clothing before reuse. |
| Ingestion: | Call a poison control center. If swallowed, do not induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. Get medical aid at once. |
| Inhalation: | Remove from exposure and move to fresh air immediately. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical aid. |
| Notes to Physician: | Treat symptomatically and supportively. |
| General Information: |
As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. |
| Extinguishing Media: |
For small fires, use dry chemical, carbon dioxide, water spray or alcohol-resistant foam. |
| Autoignition Temperature: | Not applicable. |
| Flash Point: | Not applicable. |
| NFPA Rating: |
CAS# 7732-18-5: Health- 0, Flammability- 0, Instability- 0. CAS# 34487-61-1: Health- 2, Flammability- 1, Instability- 0. |
| Explosion Limits: | Lower: n/a Upper: n/a |
General Information:
Use proper personal protective equipment as indicated in Section 8.
Spills/Leaks:
Absorb spills with absorbent (vermiculite, sand, fuller's earth) and place in plastic bags for later disposal.
Handling:
Wash thoroughly after handling. Use with adequate ventilation. Avoid contact with skin and eyes. Avoid ingestion and inhalation.
Storage:
Store in a cool, dry area. Keep containers tightly closed.
Engineering Controls:
Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower. Use adequate ventilation to keep airborne concentrations low.
Exposure Limits:
| Chemical Name: | ACGIH | NIOSH | OSHA |
| Water | None listed | None listed | None listed |
| Phenol Red, Sodium Salt | None listed | None listed | None listed |
OSHA Vacated PELs: None.
Personal Protective Equipment
Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166.
Skin: Wear appropriate gloves to prevent skin exposure.
Clothing: Wear appropriate protective clothing to prevent skin exposure.
Respirators:
Follow the OSHA respirator regulations found in 29 CFR 1910.134 or European Standard EN 149.
Use a NIOSH/MSHA or European Standard EN 149 approved respirator if exposure limits are exceeded or if irritation or other symptoms are experienced.
| Physical State: | Liquid |
| Color: | Red to orange |
| Odor: | Odorless |
| pH: | 7-8 |
| Vapor Pressure: | Not available |
| Vapor Density: | Not available |
| Evaporation Rate: | Not available |
| Viscosity: | Not available |
| Boiling Point: | Not available |
| Freezing/Melting Point: | Not available |
| Decomposition Temperature: | 285°C |
| Solubility in water: | Soluble |
| Specific Gravity/Density: | 1.0 |
| Molecular Formula: | Not applicable |
| Molecular Weight: | Not applicable |
| Chemical Stability: | Stable under normal temperatures and pressures. |
| Conditions to Avoid: | Incompatible materials, excess heat. |
| Incompatibilities with Other Materials: | Strong oxidizing agents. |
| Hazardous Decomposition Products: | Sulfur dioxide, carbon monoxide, carbon dioxide. |
| Hazardous Polymerization: | Has not been reported. |
RTECS:
CAS# 7732-18-5: ZC0110000.
CAS# 34487-61-1: unlisted.
LD50/LC50:
CAS# 7732-18-5:
Oral, rat: LD50 = >90 mL/kg.
CAS# 34487-61-1:
No information found.
Carcinogenicity:
CAS# 7732-18-5: Not listed as a carcinogen by ACGIH, IARC, NIOSH, NTP, OSHA, or CA Prop 65.
CAS# 34487-61-1: Not listed as a carcinogen by ACGIH, IARC, NIOSH, NTP, OSHA, or CA Prop 65.
Epidemiology:
No information found
Teratogenicity:
No information found
Reproductive:
No information found
Mutagenicity:
No information found
Neurotoxicity:
No information found
No information found
Dispose of in accordance with Federal, State, and local regulations.
Road/Rail (ADR/RID)
Proper Shipping Name: Mixture of Reagent (Phenol Red, Sodium Salt)
ADR UN No.: UN3288
ADR Packing Group: III ADR subrisk: -
ADR Flashpoint: - NA
Sea (IMDG)
Proper Shipping Name: Mixture of Reagent (Phenol Red, Sodium Salt)
IMDG UN No.: UN3288 IMDG Hazard Class.: 6.1
IMDG Pack Group.: III IMDG EmS: F-A, S-A
Air (ICAO/IATA)
Proper Shipping Name: Mixture of Reagent (Phenol Red, Sodium Salt)
ICAO Un No.: UN3288 ICAO Hazard Class.: 6.1
ICAO Packing Group.: III ICAO subrisk: -
ICAO Flashpoint: - N A
US Federal
TSCA:
CAS# 7732-18-5 is listed on the TSCA Inventory.
CAS# 34487-61-1 is listed on the TSCA Inventory.
SARA Reportable Quantities (RQ):
None of the components are on this list.
CERCLA/SARA Section 313:
None of the components are on this list.
OSHA - Highly Hazardous:
None of the chemicals in this product are considered highly hazardous by OSHA.
US State
State Right to Know:
None of the components are on this list.
California Regulations:
None.
European/International Regulations
Canadian DSL/NDSL:
CAS# 7732-18-5 is listed on Canada's DSL List.
CAS# 34487-61-1 is listed on Canada's DSL List.
Canada Ingredient Disclosure List:
CAS# 7732-18-5 is not listed on Canada's Ingredient Disclosure List.
CAS# 34487-61-1 is not listed on Canada's Ingredient Disclosure List.
Information in this MSDS is from available published sources and is believed to be accurate. No warranty, express or implied, is made and LabChem Inc. assumes no liability resulting from the use of this MSDS. The user must determine suitability of this information for his application.
DISCLAIMER OF LIABILITY :The information in this SDS was obtained from sources which we believe are reliable. However, the information is provided without any warranty, express or implied, regarding its correctness. The conditions or methods of handling, storage, use or disposal of the product are beyond our control and may be beyond our knowledge. For this and other reasons, we do not assume responsibility and expressly disclaim liability for loss, damage or expense arising out of or in any way connected with the handling, storage, use or disposal of the product. This MSDS was prepared and is to be used only for this product. If the product is used as a component in another product, this MSDS information may not be applicable.
Availability
Phenol Red Indicator Solution manufacturer supplier distributor in Mumbai, Kandla, Kolkata, Vizag, Chennai, India, Fujairah, Dubai UAE, Muscat Oman, Kenya Africa. Get the best quality of Phenol Red Indicator Solution at a competitive price from us. We have ready stock of Phenol Red Indicator Solution in India, UAE Gulf, Oman, Kenya Africa. Contact us for bulk as well as small orders.
