Answer
Deparaffinise to 95% alcohol, stain in 0.1% LFB at 56–60 °C overnight (or 2 h), rinse in 95% alcohol and water, differentiate in 0.05% lithium carbonate then 70% alcohol until grey and white matter separate, counterstain with cresyl violet, dehydrate and mount.
Detailed question
How is Luxol fast blue staining done?
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