LUXOL FAST BLUE
- Part number
- RXSOL-60-6604-866
- Category
- Lab Chemical
- Brand
- RXSOL
Overview
Luxol Fast Blue Stain Kit (Myelin Stain) ab150675 is designed for staining myelin/myelinated axons and Nissl bodies on formalin fixed, paraffin-embedded tissue as well as frozen tissue. This product is used for identifying the basic neuronal structure in brain or spinal cord sections.
Applications
Luxol fadt blue stain (LFB) is used to visualize myelin in nerve tissue. Thanks to this staining, it is possible to visualize the myelin and the fatty substance of the brain called white matter which includes the axons of the nerve cells. The LFB coloration thus makes it possible to differentiate the white matter from the gray matter.
Characteristics and notes
Luxol fast blue is a copper phthalocyanine dye that is soluble in alcohol and is attracted to bases found in the lipoproteins of the myelin sheath. Under the stain, myelin fibers appear blue, neuropil appears pink, and nerve cells appear purple.
Availability
Frequently asked questions
How is Luxol fast blue staining done?
Deparaffinise to 95% alcohol, stain in 0.1% LFB at 56–60 °C overnight (or 2 h), rinse in 95% alcohol and water, differentiate in 0.05% lithium carbonate then 70% alcohol until grey and white matter separate, counterstain with cresyl violet, dehydrate and mount.
Is a kit available?
Yes — LFB solution, lithium carbonate differentiator and cresyl-violet counterstain as a myelin stain kit on request.
Pack sizes?
5 g, 25 g powder; 100 ml, 500 ml solution.
