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Test Kit For Water

H2S Vial (Bacteriological ) Kit

The H2S vial kit is a one-bottle screening test for the bacteriological quality of drinking water, based on the hydrogen-sulphide paper-strip method (Manja et al., 1982). Faecal and enteric bacteria such as Salmonella, Proteus and Citrobacter produce H2S, which blackens the iron-containing medium. The vial contains sterile dry medium; fill with the sample to the mark, shake, and keep at 30–37 °C (incubator, or simply a shirt pocket overnight) for 18 hours. No colour change — fit to drink; black — contaminated. No laboratory, chlorine removal or volume measurement is needed; one pack is one test.
Part number
RXSOL-62-5540-001
Brand
RXSOL
H2S Vial (Bacteriological ) Kit

Overview

H2S vial (Bacteriological ) Kit use for checking microbiological quality of water. Water borne diseases like typhoid ,cholera, diarrhoea and jaundice and caused  by polluted water supply. The conventional method for testing the microbiological quality of water needs the service of  a qualified microbiologist , laboratory facilities  and take 60 - 72 hours to know the result. And further the laboratory may charge any amount per sample for the test. The new devloped screening test ,  done at field itself, dispenses with all these problems and will cost  & timing much less.

Technical specifications

H2S KITS FOR WATER SAMPLE TEST. (H2SKIT FOR BACTERIOLOGICAL TEST FOR DRINKING WATER)

Applications

H2S vials test of water.
What is H2S Strip Test Kit?
RXSOL  H2S Strip Test is ready to use simple device based on hydrogen sulphide indicator bacteria for carrying out bacteriological examination of drinking water. The Hydrogen Sulphide Paper Strip Method.

The Hydrogen Sulphide Paper Strip Method developed by Manja et al. (1982) is an on site testing method for the microbial quality of drinking water and is based on the detection of hydrogen sulphide producing bacteria rather than the coliform bacteria. Human faeces contain a high concentration of sulphate reducing bacteria of up to 1010/g (Levett, 1993) and some of the enteric bacteria such as Salmonella, Proteus, Citrobacter and some strains of lebsiella,also produce hydrogen sulphide. 
Sterlized paper strip treated with media is kept in sterlized glass bottle. The water sample is filled in the bottle and kept for 24 to 48 hours at room temperature (25 to 37 C).
If bacteria are present in the sample, they produce hydrogen sulphide, which turns water sample black.

Dosage and directions

Method of Testing Water :

Dry and sterile media are provided in nthe screw capped bottle which are ready for use. Fill the water to be tested up to the upper level mark of the bottle. Shake the bottle gently. After 5 minute keep the bottle at room temperature of 30 to 35°C or in the incubator at 37°C  or in curdomatic for 18 hrs. If  none of these are available the bottle can kept in the pocket while sleeping in the night.

The following change may be observed -

1. No change in colour of water in the bottle  / or Change in yellow  ting  - Water is fit for drinking

2. Water in bottle  turn black   - Water is unfit for drinking . Water may have disease causing bacteria. Hence it has to be boiled or chlorinated before drinking.

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Features of H2S Strip Test Kit-
 
1) Ready to use: The water sample (20 ml) from the source can be directly taken in to the pressurized H2S Strip Test bottle. Presterlized bottle contains premeasured sterile media on paper strip. There is no need of preparation and sterilization of media or sterilization of bottle. Bacteriological test of sample water (20 ml) starts at sample source.
2) Simplicity: H2S Strip test kit's bacteriological test is so simple that even a layman can perform it. For the reason it is most suited for rural & remote areas and for those who do not have infrastructure to carry out standard microbiological tests.
3) Economical: H2S Strip test kit is most economical proposition. It does not require any set up or laboratory or services of microbiologist.
4) Portability: H2S Strip Test Kit is made up of glass, light in weight, easy to carry anywhere, anytime.
5) Disposable: Destroy after use.
6) Confidence: Results are reliable, meaningful and reproducible.
7) Illustrative Direction: Simple to follow illustrative direction are given which explains the use of the test kit.
 
 


 

 
Features of H2S Strip Test Kit-
 
1) Ready to use: The water sample (20 ml) from the source can be directly taken in to the pressurized H2S Strip Test bottle. Presterlized bottle contains premeasured sterile media on paper strip. There is no need of preparation and sterilization of media or sterilization of bottle. Bacteriological test of sample water (20 ml) starts at sample source.
2) Simplicity: H2S Strip test kit's bacteriological test is so simple that even a layman can perform it. For the reason it is most suited for rural & remote areas and for those who do not have infrastructure to carry out standard microbiological tests.
3) Economical: H2S Strip test kit is most economical proposition. It does not require any set up or laboratory or services of microbiologist.
4) Portability: H2S Strip Test Kit is made up of glass, light in weight, easy to carry anywhere, anytime.
5) Disposable: Destroy after use.
6) Confidence: Results are reliable, meaningful and reproducible.
7) Illustrative Direction: Simple to follow illustrative direction are given which explains the use of the test kit.

Characteristics and notes

Advantage of the test :

  • It is a field test , hence water sample can directly be collected from the tap.
  • No need to remove the chlorine in chlorinated water since the content of the bottle instantaneously remove it.
  • No need the messure the volume of water as the level of label marked indicated in bottle.
  • The test is simple , rapid and inexpensive.
  • The test can be done even by uneducated person in field. 

Safety and SDS

Read the safety data sheet before handling. Wear suitable gloves and eye protection.

View safety data sheet

PRODUCT DATA SHEET
H2S TEST STRIP KIT  
For rapid detection and confirmation of Salmonella and Citrobacter in potable water •Easy to perform
•User-friendly
•Screening tests
•Low-cost rapid test
•Suitable for handling by untrained personnel for monitoring of rural drinking water sources

Kit contains
•Glass vial containing H2S test strip
*Store in a dry place, below 25°C and protect from direct Sunlight.

Instructions for use
Carefully open the bottle cap and fill the water sample up to the arrow mark. Quickly re-cap the test bottle and secure it tightly. Shake gently for few minutes to allow the medium to diffuse in water evenly. Incubate at 32 - 37ºC for 18 - 48 hours and take observation.

Observation Broth colour turns to black from initial light amber colour – Salmonella present Water is not suitable for drinking

Principle There is a need for rapid methods to quickly determine the pathogenic bacteria in water other than coliforms. Monitoring microbiological quality of drinking water also needs examination of water samples for presence of bacterialike Salmonella and Citrobactor freundiiusing rapid kits. H2S test strip kit contains media-impregnated paper strip containingall the essential nutrients for the growth of Salmonella and Citrobactor freundii. Presumptively,presence of these bacteriais indicated by H2S production, resulting in colour change of broth to black from initial light amber colour.

Microbiological parameters (Growth promotion test) Culture characteristics observed after inoculation (10-100 CFU) and incubation at 32 - 37°C for 18 – 48 hours.

Mail us : To get H2s discounted price        --- rxsolchemicals@gmail.com  

Test strains ATCC Inoculum  Growth Colour of the medium
Salmonella typhimurium 14028 10-100 Luxuriant Black
Citrobacter freundii 8090  10-100 Luxuriant Black
Escherichia coli  25922  10-100  Luxuriant  No change
         
Stock Point  Mumbai, Kandla Chennai Visakhapatnam Kolkata, Fujairah
 
 

Land transport (ADR): NOT REGULATED FOR TRANSPORT OF DANGEROUS GOODS 

UN        Number                                          Not Applicable
UN Proper shipping name Not Applicable
Transport Hazard Class Not Applicable
Transport Hazard Subrisk Not Applicable
Packing group Not Applicable
Environmental hazard Not Applicable
Special precautions for user : - Hazard identification (Kemler) : Not Applicable
Classification code                   : Not Applicable
Hazard Label                            : Not Applicable
Special provisions                    : Not Applicable
Limited quantity                        : Not Applicable
Tunnel Restriction Code           : Not Applicable

 Air transport (ICAO-IATA / DGR): NOT REGULATED FOR TRANSPORT OF DANGEROUS GOODS

UN        Number                                          Not Applicable
UN Proper shipping name Not Applicable
Transport Hazard Class ICAO/IATA Class            : Not Applicable
ICAO / IATA Subrisk       : Not Applicable
ERG Code                      : Not Applicable
Transport Hazard Subrisk Not Applicable
Packing group Not Applicable
Environmental hazard Not Applicable
Special precautions for user : - Special provisions                                           : Not Applicable
Cargo Only Packing Instructions                     : Not Applicable
Cargo Only Maximum Qty / Pack                    : Not Applicable
Passenger and Cargo Packing Instructions     : Not Applicable
Passenger and Cargo Maximum Qty / Pack    : Not Applicable
Passenger and Cargo Limited Quantity
Packing Instructions                                         : Not Applicable
Passenger and Cargo Limited
Maximum Qty / Pack                                        : Not Applicable

 Sea transport (IMDG-Code / GGVSee): NOT REGULATED FOR TRANSPORT OF DANGEROUS GOODS

UN        Number                                          Not Applicable
UN Proper shipping name Not Applicable
IMDC Class Not Applicable
IMDG Subrisk Not Applicable
Packing group Not Applicable
Environmental hazard Not Applicable
Special precautions for user : - EMS Number            : Not Applicable
Special provisions     : Not Applicable
Limited Quantities     : Not Applicable

 Inland waterways transport (ADN): NOT REGULATED FOR TRANSPORT OF DANGEROUS GOODS

UN        Number                                          Not Applicable
UN Proper shipping name Not Applicable
Transport Hazard Class Not Applicable
Transport Hazard Subrisk Not Applicable
Packing group Not Applicable
Environmental hazard Not Applicable
Special precautions for user : - Classification code  : Not Applicable
Special provisions   : Not Applicable
Limited quantity Not : Applicable
Equipment required : Not Applicable
Fire cones number  : Not Applicable

Request the signed SDS as PDF

Availability

H2S Vial (Bacteriological ) Kit manufacturer supplier distributor in Mumbai, Kandla, Kolkata, Vizag, Chennai, India, Fujairah, Dubai, Sharjah, UAE, Gulf, Middle East, Muscat Oman, Kenya Africa. Get the best quality of H2S Vial (Bacteriological ) Kit at a competitive price from us. We have ready stock of H2S Vial (Bacteriological ) Kit in India, UAE Gulf, Oman, Kenya Africa. Contact us for bulk as well as small orders.

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Frequently asked questions

How does the H2S strip/vial test work?

H2S-producing bacteria associated with faecal pollution reduce the medium and form black iron sulphide; it detects the same contamination as a coliform test, faster and in the field.

What do I do if the water turns black?

Disinfect: about 1 lb of granular calcium hypochlorite per 10,000 gallons (≈5 ppm), hold 30 minutes, retest.

Is it a quantitative count?

No — presence/absence; use the Coliform Test Kit PUT-n-SEE for an MPN estimate.